Simultaneous sizing and refractive index analysis of heterogeneous nanoparticle suspensions
In a collaboration with Matz Liebel from Niek Van Hulst's group (ICFO), we developed a straightforward digital holographic platform termed holographic nanoparticle tracking (holoNTA) that characterises heterogenous nanoparticle suspensions by simultaneously quantifying the random motion and the amount of light each nanoparticle scatters.
We show that the increase in imaging volume granted by digital holography directly translates into longer trajectories, which allows measuring the intrinsic heterogeneity of the nanoparticle samples, a feat inaccessible to other approaches. We benchmark our approach with both experiments and simulations and show that holoNTA has the sensitivity to detect minute changes in nanoparticle properties upon interaction with proteins, i.e. protein corona formation.
Reference
Unai Ortiz-Orruño, Romain Quidant, Niek F. van Hulst, Matz Liebel, and Jaime Ortega Arroyo. Simultaneous Sizing and Refractive Index Analysis of Heterogeneous Nanoparticle Suspensions. ACS Nano 2023 17 (1), 221-229. doi: external page 10.1021/acsnano.2c06883